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Rabbit anti-ATP6V1H Antibody, Affinity Purified - A304-308A-T - 10 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-ATP6V1H Antibody, Affinity Purified
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Size: 10 µg
£89.00
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Catalogue number:
A304-308A-T
Name:
Rabbit anti-ATP6V1H Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to ATP6V1H immobilized on solid support.The epitope recognized by A304-308A-T maps to a region between residue 400 to 450 of human ATPase, H+ Transporting, Lysosomal 50/57kDa, V1 Subunit H using the numbering given in entry NP_057025.2 (GeneID 51606).Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP

Product Type:
Antibodies
Alternative Names:
ATPase, H+ transporting, lysosomal 50/57kDa, V1 subunit H, CGI-11, MSTP042, NBP1, nef-binding protein 1, protein VMA13 homolog, SFD, SFDalpha, SFDbeta, vacuolar ATP synthase subunit H, vacuolar ATPase subunit H, vacuolar proton pump subunit H, vacuolar pr
Species Reactivity:
Human, Mouse
Immunogen:
Between 400 and 450
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human and mouse ATP6V1H by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-ATP6V1H antibody A304-308A-T (lot A304-308A-T-1) used for WB at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

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Detection of human ATP6V1H by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-ATP6V1H antibody A304-308A-T (lot A304-308A-T-1) used for IP at 6 µg per reaction. ATP6V1H was also immunoprecipitated by rabbit anti-ATP6V1H antibody BL15221. For blotting immunoprecipitated ATP6V1H, A304-308A-T was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

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