Antibody was affinity purified using an epitope specific to KIF5B immobilized on solid support. The epitope recognized by A304-306A-T maps to a region between residue 675 to 725 of human Kinesin Family Member 5B using the numbering given in entry NP_004512.1 (GeneID 3799). Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
conventional kinesin heavy chain, epididymis secretory protein Li 61, HEL-S-61, kinesin 1 (110-120kD), kinesin heavy chain, kinesin-1 heavy chain, KINH, KNS, KNS1, ubiquitous kinesin heavy chain, UKHC
Between 675 and 725
2 - 8°C
Detection of human and mouse KIF5B by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, and mouse TCMK-1 cells. Antibodies: Affinity purified rabbit anti-KIF5B antibody A304-306A-T (lot A304-306A-T-1) used for WB at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of human KIF5B by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T and cells. Antibodies: Affinity purified rabbit anti-KIF5B antibody A304-306A-T (lot A304-306A-T-1) used for IP at 6 µg per reaction. KIF5B was also immunoprecipitated by rabbit anti-KIF5B antibody A304-307A. For blotting immunoprecipitated KIF5B, A304-307A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 1 second.