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Rabbit anti-SHMT1 Antibody, Affinity Purified - A304-259A-T - 10 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-SHMT1 Antibody, Affinity Purified
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Size: 10 µg
£89.00
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Catalogue number:
A304-259A-T
Name:
Rabbit anti-SHMT1 Antibody, Affinity Purified
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Availability:

Antibody was affinity purified using an epitope specific to SHMT1 immobilized on solid support.The epitope recognized by A304-259A-T maps to a region between residue 453 to 483 of human Serine Hydroxymethyltransferase 1 using the numbering given in entry NP_004160.3 (GeneID 6470).Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP, IHC

Product Type:
Antibodies
Alternative Names:
CSHMT, cytoplasmic serine hydroxymethyltransferase, glycine hydroxymethyltransferase, serine hydroxymethyltransferase 1 (soluble), serine hydroxymethyltransferase, cytosolic, serine methylase, SHMT
Species Reactivity:
Human, Mouse
Immunogen:
Between 453 and 483
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human SHMT1 by western blot. Samples: Whole cell lysate (50 µg) from Jurkat cells. Antibodies: Affinity purified rabbit anti-SHMT1 antibody A304-259A-T (lot A304-259A-T-1) used for WB at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

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Detection of mouse SHMT1 by western blot. Samples: Whole cell lysate (50 µg) from NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-SHMT1 antibody A304-259A-T (lot A304-259A-T-1) used for WB at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

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Detection of human SHMT1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti-SHMT1 antibody A304-259A-T (lot A304-259A-T-1) used for IP at 6 µg per reaction. SHMT1 was also immunoprecipitated by rabbit anti-SHMT1 antibody BL15560. For blotting immunoprecipitated SHMT1, A304-259A-T was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

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Detection of human SHMT1 by immunohistochemistry. Samples: FFPE section of human lung cancer. Antibody: Affinity purified rabbit anti- SHMT1 (Cat. No. A304-259A-T Lot1) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB

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Detection of mouse SHMT1 by immunohistochemistry. Samples: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti- SHMT1 (Cat. No. A304-259A-T Lot1) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB

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