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Rabbit anti-EZH2 Antibody, Affinity Purified - A304-197A-T - 10 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-EZH2 Antibody, Affinity Purified
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Size: 10 µg
£89.00
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Catalogue number:
A304-197A-T
Name:
Rabbit anti-EZH2 Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to EZH2 immobilized on solid support.The epitope recognized by A304-197A-T maps to a region between residue 696 to 746 of human Enhancer of Zeste Homolog 2 using the numbering given in entry CAA64955.1 (GeneID 2146).Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP, IHC

Product Type:
Antibodies
Alternative Names:
enhancer of zeste homolog 2, ENX1, ENX-1, EZH2b, histone-lysine N-methyltransferase EZH2, KMT6, KMT6A, lysine N-methyltransferase 6, WVS, WVS2
Species Reactivity:
Human, Mouse
Immunogen:
Between 696 and 746
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human and mouse EZH2 by western blot. Samples: Whole cell lysate (50 µg) from 293T, HeLa, Jurkat, and mouse TCMK-1 cells. Antibodies: Affinity purified rabbit anti-EZH2 antibody A304-197A-T (lot A304-197A-T-1) used for WB at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

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Detection of human EZH2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-EZH2 antibody A304-197A-T (lot A304-197A-T-1) used for IP at 6 µg/mg lysate. EZH2 was also immunoprecipitated by rabbit anti-EZH2 antibody A304-196A. For blotting immunoprecipitated EZH2, A304-197A-T was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

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Detection of human EZH2 by immunohistochemistry. Samples: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- EZH2 (Cat. No. A304-197A-T Lot1) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB

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Detection of mouse EZH2 by immunohistochemistry. Samples: FFPE section of mouse plasmacytoma. Antibody: Affinity purified rabbit anti- EZH2 (Cat. No. A304-197A-T Lot1) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB

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