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Rabbit anti-IDH1 Antibody, Affinity Purified - A304-162A-T - 10 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-IDH1 Antibody, Affinity Purified
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Size: 10 µg
£89.00
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Catalogue number:
A304-162A-T
Name:
Rabbit anti-IDH1 Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to IDH1 immobilized on solid support.The epitope recognized by A304-162A-T maps to a region between residue 364 to 414 of human Isocitrate Dehydrogenase 1 using the numbering given in entry NP_005887.2 (GeneID 3417).Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP

Product Type:
Antibodies
Alternative Names:
Cytosolic NADP-isocitrate dehydrogenase, epididymis luminal protein 216, epididymis secretory protein Li 26, epididymis secretory sperm binding protein, HEL-216, HEL-S-26, IDCD, IDH, IDP, IDPC, isocitrate dehydrogenase [NADP] cytoplasmic, isocitrate dehyd
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Between 364 and 414
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human, mouse and rat IDH1 by western blot. Samples: Whole cell lysate (50 µg) from K-562, HeLa, HEK293T, mouse TCMK-1, mouse NIH 3T3, and rat C6 cells. Antibodies: Affinity purified rabbit anti-IDH1 antibody A304-162A-T (lot A304-162A-T-1) used for WB at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

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Detection of human IDH1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti-IDH1 antibody A304-162A-T (lot A304-162A-T-1) used for IP at 6 µg/mg lysate. IDH1 was also immunoprecipitated by rabbit anti-IDH1 antibody A304-161A. For blotting immunoprecipitated IDH1, A304-162A-T was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

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