Antibody was affinity purified using an epitope specific to CaMKK2 immobilized on solid support. The epitope recognized by A304-009A-T maps to a region between residue 538 to 588 of human Calcium/Calmodulin-Dependent Protein Kinase Kinase 2, Beta using the numbering given in entry NP_006540.3 (GeneID 10645). Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
calcium/calmodulin-dependent protein kinase beta, calcium/calmodulin-dependent protein kinase kinase 2, calcium/calmodulin-dependent protein kinase kinase 2, beta, Calcium/calmodulin-dependent protein kinase kinase beta, caM-kinase kinase 2, caM-kinase ki
Between 538 and 588
2 - 8°C
Detection of human CaMKK2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-CaMKK2 antibody A304-009A-T (lot A304-009A-T-1) used for IP at 6 µg/mg lysate. For blotting immunoprecipitated CaMKK2, A304-009A-T was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.