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Rabbit anti-PSME3 Antibody, Affinity Purified - A303-879A-T - 10 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-PSME3 Antibody, Affinity Purified
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Size: 10 µg
£89.00
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Catalogue number:
A303-879A-T
Name:
Rabbit anti-PSME3 Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to PSME3 immobilized on solid support.The epitope recognized by A303-879A-T maps to a region between residue 204 and 254 of human Proteasome (Prosome, Macropain) Activator Subunit 3 using the numbering given in entry NP_005780.2 (GeneID 10197).Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP, IHC

Product Type:
Antibodies
Alternative Names:
11S regulator complex gamma subunit, 11S regulator complex subunit gamma, activator of multicatalytic protease subunit 3, epididymis secretory protein Li 283, HEL-S-283, Ki, Ki antigen, Ki nuclear autoantigen, PA28 gamma variant 5, PA28G, PA28gamma, PA28-
Species Reactivity:
Human, Mouse
Immunogen:
Between 204 and 254
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human and mouse PSME3 by western blot. Samples: Whole cell lysate from HEK293T (15 and 50 µg), HeLa (50µg), Jurkat (50µg), and mouse NIH 3T3 (50µg) cells. Antibodies: Affinity purified rabbit anti-PSME3 antibody A303-879A-T (lot A303-879A-T-1) used for WB at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

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Detection of human PSME3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-PSME3 antibody A303-879A-T (lot A303-879A-T-1) used for IP at 6 µg/mg lysate. For blotting immunoprecipitated , A303-879A-T was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

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Detection of human PSME3 by immunohistochemistry. Samples: FFPE section of human non-small cell lung cancer. Antibody: Affinity purified rabbit anti- PSME3 (Cat. No. A303-879A-T Lot1) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB

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Detection of mouse PSME3 by immunohistochemistry. Samples: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti- PSME3 (Cat. No. A303-879A-T Lot1) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB

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