Antibody was affinity purified using an epitope specific to RPA32 immobilized on solid support. The epitope recognized by A303-874A-T maps to a region between residue 220 and 270 of human Replication Protein A2 (32kD) using the numbering given in entry NP_002937.1 (GeneID 6118). Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
REPA2, replication factor A protein 2, replication protein A 32 kDa subunit, replication protein A 34 kDa subunit, RF-A protein 2, RP-A p32, RP-A p34, RPA32
Between 220 and 270
2 - 8°C
Detection of human RPA32 by western blot. Samples: Whole cell lysate from HEK293T (15 and 50 µg), HeLa (50µg), and Jurkat (50µg) cells. Antibodies: Affinity purified goat anti-RPA32 antibody A303-874A-T (lot A303-874A-T-1) used for WB at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of human RPA32 by immunohistochemistry. Sample: FFPE section of human stomach carcinoma. Antibody: Affinity purified rabbit anti- RPA32 (Cat. No. A303-874A Lot1) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB