Antibody was affinity purified using an epitope specific to Lasu1/Ureb1 immobilized on solid support. The epitope recognized by A303-836A-T maps to a region between residue 2250 and 2300 of human HECT domain protein LASU1/Upstream Regulatory Element Binding Protein 1 using the numbering given in entry NP_113584.3 (GeneID 10075). Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
ARF-binding protein 1, ARF-BP1, BJ-HCC-24 tumor antigen, E3 ubiquitin-protein ligase HUWE1, HECT domain protein LASU1, HECT, UBA and WWE domain-containing protein 1, HECTH9, HECT-type E3 ubiquitin transferase HUWE1, homologous to E6AP carboxyl terminus ho
Between 2250 and 2300
2 - 8°C
Detection of human Lasu1/Ureb1 by western blot. Samples: Whole cell lysate from HEK293T (15 and 50 µg) and HeLa (H; 50 µg) cells. Antibodies: Affinity purified goat anti-Lasu1/Ureb1 antibody A303-836A-T (lot A303-836A-T-1) used for WB at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.