Antibody was affinity purified using an epitope specific to ARVCF immobilized on solid support. The epitope recognized by A303-310A-T maps to a region between residue 75 and 125 of human Armadillo Repeat Gene Deleted in Velocardiofacial Syndrome using the numbering given in entry NP_001661.1 (GeneID 421). Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
armadillo repeat gene deleted in velocardiofacial syndrome, armadillo repeat protein deleted in velo-cardio-facial syndrome
between 75 and 125
2 - 8°C
Detection of human ARVCF by western blot. Samples: Whole cell lysate from Jurkat (15 and 50 µg), HEK293T (T; 50 µg) and HeLa (H; 50 µg) cells. Antibodies: Affinity purified rabbit anti-ARVCF antibody A303-310A-T used for WB at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.