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Rabbit anti-Bin1 Antibody, Affinity Purified - A302-729A-T - 10 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-Bin1 Antibody, Affinity Purified
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Size: 10 µg
£89.00
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Catalogue number:
A302-729A-T
Name:
Rabbit anti-Bin1 Antibody, Affinity Purified
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Availability:

Antibody was affinity purified using an epitope specific to Bin1 immobilized on solid support.The epitope recognized by A302-729A-T maps to a region between residue 543 and 593 of human Bridging Integrator 1 using the numbering given in entry NP_647593.1 (GeneID 274).Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP, IHC

Product Type:
Antibodies
Alternative Names:
AMPH2, amphiphysin II, amphiphysin-like protein, AMPHL, box dependant MYC interacting protein 1, box-dependent myc-interacting protein 1, Bridging integrator 1, CNM2, myc box-dependent-interacting protein 1, SH3P9
Species Reactivity:
Human
Immunogen:
Between 543 and 593
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human Bin1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-Bin1 antibody A302-729A-T (lot A302-729A-T-2) used for WB at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

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Detection of human Bin1 by immunohistochemistry. Samples: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-Bin1 (Cat. No. A302-729A-T Lot2) used at a dilution of 1:1,000 (1µg/ml) Detection: DAB

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Detection of human Bin1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Bin1 antibody A302-729A-T (lot A302-729A-T-2) used for IP at 6 µg per reaction. Bin1 was also immunoprecipitated by rabbit anti-Bin1 antibody A302-728A. For blotting immunoprecipitated Bin1, A302-729A-T was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

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