Antibody was affinity purified using an epitope specific to Cyclin Y immobilized on solid support. The epitope recognized by A302-376A-T maps to a region between residue 291 and 441 of human cyclin Y using the numbering given in entry NP_659449.3 (GeneID 219771). Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
C10orf9, CBCP1, CCNX, CFP1, cyclin box protein 1, cyclin fold protein 1, cyclin-box carrying protein 1, cyclin-X, cyclin-Y, cyc-Y
between 291 and 341
2 - 8°C
Detection of human Cyclin Y by western blot. Samples: Whole cell lysate (50 µg) from HeLa (H), HEK293T (T), mouse NIH 3T3 (3), and MCF-7 (M) cells. Antibodies: Affinity purified rabbit anti-Cyclin Y antibody A302-376A-T used for WB at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.