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Antibodies for Immuno-Oncology Research

Bromodomain-Containing (BRD) Antibodies
Achieve High Yield Mammalian Protein Production In Suspension HEK 293 & CHO Derived Cells

Rabbit anti-BRCA1 Antibody, Affinity Purified - A301-377A-T - 10 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-BRCA1 Antibody, Affinity Purified
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Size: 10 µg
£89.00
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Catalogue number:
A301-377A-T
Name:
Rabbit anti-BRCA1 Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to BRCA1 immobilized on solid support.The epitope recognized by A301-377A-T maps to a region between residue 250 and 300 of human breast cancer 1 using the numbering given in entry NP_009225.1 (GeneID 672).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP, IHC

Product Type:
Antibodies
Alternative Names:
BRCA1/BRCA2-containing complex, subunit 1, BRCAI, BRCC1, breast and ovarian cancer susceptibility protein 1, breast cancer 1, early onset, breast cancer type 1 susceptibility protein, BROVCA1, early onset breast cancer 1, Fanconi anemia, complementation g
Species Reactivity:
Human
Immunogen:
Between 250 and 300
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human BRCA1 by western blot. Samples: Whole cell lysate (5, 15 and 50 µg) from HeLa cells. Antibodies: Affinity purified rabbit anti-BRCA1 antibody A301-377A-T (lot A301-377A-T-2) used for WB at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

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Detection of human BRCA1 by immunohistochemistry. Samples: FFPE section of human breast carcinoma (left) and ovarian carcinoma (right). Antibody: Affinity purified rabbit anti-BRCA1 (Cat. No. A301-377A-T Lot2) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB

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Detection of human BRCA1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-BRCA1 antibody A301-377A-T (lot A301-377A-T-2) used for IP at 6 µg/mg lysate. BRCA1 was also immunoprecipitated by a previous lot of this antibody (lot A301-377A-1) and rabbit anti-BRCA1 antibody A301-378A. For blotting immunoprecipitated BRCA1, A301-377A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

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