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Rabbit anti-BRCA1 Antibody, Affinity Purified

Catalogue number:
A301-377A-T
Size:
10 µl (1000 µg/ml) _$$_
Product is available in:
  • UK
  • Ireland
  • Europe
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  • Rest of World
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Applications:

WB, IP, IHC

Target:

BRCA1

Species Reactivity:

Human

Antibody Type:

Polyclonal

Immunogen:

between 250 and 300

Alternative Names:

IRIS; RING-type E3 ubiquitin transferase BRCA1; BRCAI; RING finger protein 53; RNF53; PSCP; protein phosphatase 1, regulatory subunit 53; PPP1R53; PNCA4; BRCA1/BRCA2-containing complex, subunit 1; breast and ovarian cancer susceptibility protein 1; BRCC1;

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Product Description:

Rabbit anti-BRCA1 Antibody, Affinity Purified - 10 µl (1000 µg/ml). Host: Rabbit. Conjugate Type: Unconjugated.

Storage Temperature:

2 - 8°C

Detection of human BRCA1 by western blot
Detection of human BRCA1 by western blot. Samples: Whole cell lysate (5, 15 and 50 µg) from HeLa cells. Antibodies: Affinity purified rabbit anti-BRCA1 antibody A301-377A-T (lot A301-377A-T-2) used for WB at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of human BRCA1 by immunohistoc
Detection of human BRCA1 by immunohistochemistry. Samples: FFPE section of human breast carcinoma (left) and ovarian carcinoma (right). Antibody: Affinity purified rabbit anti-BRCA1 (Cat. No. A301-377A-T Lot2) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB
Detection of human BRCA1 by western blot
Detection of human BRCA1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-BRCA1 antibody A301-377A-T (lot A301-377A-T-2) used for IP at 6 µg/mg lysate. BRCA1 was also immunoprecipitated by a previous lot of this antibody (lot A301-377A-1) and rabbit anti-BRCA1 antibody A301-378A. For blotting immunoprecipitated BRCA1, A301-377A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
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