Antibody was affinity purified using an epitope specific to LIS-1 immobilized on solid support. The epitope recognized by A300-409A-T maps to a region between residue 1 and 50 of human Lissencephaly 1 using the numbering given in entry NP_000421.1 (GeneID 5048). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
LIS1, LIS-1, LIS2, lissencephaly 1 protein, Lissencephaly-1 protein, MDCR, MDS, NudF, PAF acetylhydrolase 45 kDa subunit, PAFAH, PAF-AH 45 kDa subunit, PAFAH alpha, PAF-AH alpha, platelet-activating factor acetylhydrolase 1b, regulatory subunit 1 (45kDa),
Human, Mouse, Rat
between 1 and 50
2 - 8°C
Detection of human, mouse and rat LIS-1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, K-562, mouse TCMK-1, and rat C6 cells. Antibodies: Affinity purified rabbit anti-LIS-1 antibody A300-409A-T (lot A300-409A-T-2) used for WB at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.