Antibody was affinity purified using an epitope specific to PIM1 immobilized on solid support. The epitope recognized by A300-313A-M maps to a region between residue 250 and the C-terminus (residue 313) of human PIM1 oncoprotein using the numbering given in Swiss-Prot entry P11309 (GeneID 5292).
Oncogene PIM1, PIM, pim-1 kinase 44 kDa isoform, pim-1 oncogene (proviral integration site 1), proto-oncogene serine/threonine-protein kinase pim-1, serine/threonine-protein kinase pim-1
between 250 and C-term
2 - 8°C
Detection of human PIM1 by western blot. Samples: Whole cell lysate (50 µg) from K-562 cells. Antibody: Affinity purified rabbit anti-PIM1 antibody (Cat. No. A300-313A-M) used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 20 seconds.