Antibody was affinity purified using an epitope specific to RPA32 immobilized on solid support. The epitope recognized by A300-244A-T maps to a region between residue 225 and the C-terminus (residue 270) of human Replication Protein A2 (32kD) using the numbering given in entry NP_002937.1 (GeneID 6118). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
REPA2, replication factor A protein 2, replication protein A 32 kDa subunit, replication protein A 34 kDa subunit, RF-A protein 2, RP-A p32, RP-A p34, RPA32
between 225 and C-term
2 - 8°C
Detection of human and mouse RPA32 by western blot. Samples: Whole cell lysate (5 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RPA32 antibody A300-244A-T (lot A300-244A-T-3) used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.