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High Quality Recombinant Rabbit Monoclonal Antibodies
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Rabbit anti-SMC6 Antibody, Affinity Purified - A300-237A-T - 20 µl (2 blots) - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-SMC6 Antibody, Affinity Purified
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Size: 20 µl (2 blots)
£89.00
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Catalogue number:
A300-237A-T
Name:
Rabbit anti-SMC6 Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to SMC6 immobilized on solid support.The epitope recognized by A300-237A-T maps to a region between residues 1050 and the C-terminus (residue 1091) of human Structural Maintenance of Chromosomes 6 using the numbering given in TrEMBL entry Q96SB8 (GeneID 79677).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP, IHC

Product Type:
Antibodies
Alternative Names:
hSMC6, SMC protein 6, SMC-6, SMC6 structural maintenance of chromosomes 6-like 1, SMC6L1, structural maintenance of chromosomes protein 6
Species Reactivity:
Human, Mouse
Immunogen:
Between 1050 and C-term
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human and mouse SMC6 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, MCF-7, and mouse TCMK-1 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SMC6 antibody A300-237A-T (lot A300-237A-T-3) used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.

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Detection of Human SMC6 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SMC6 antibody A300-237A-T (lot A300-237A-T-3) used for IP at 20 µl per reaction. For blotting immunoprecipitated SMC6, A300-237A-M was used at 1:1000. Detection: Chemiluminescence with an exposure time of 3 minutes.

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Detection of human SMC6 by immunohistochemistry. Sample: FFPE section of prostate carcinoma. Antibody: Rabbit anti-SMC6 antibody (A300-237A-T-3) used at 1:40. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P).

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