Rabbit anti-SMC6 Antibody, Affinity Purified - A300-237A-T - 20 µl (2 blots) - Bethyl Laboratories, Inc. - antibodies
Antibody was affinity purified using an epitope specific to SMC6 immobilized on solid support. The epitope recognized by A300-237A-T maps to a region between residues 1050 and the C-terminus (residue 1091) of human Structural Maintenance of Chromosomes 6 using the numbering given in TrEMBL entry Q96SB8 (GeneID 79677). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Applications:
WB, IP, IHC
Detection of human and mouse SMC6 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, MCF-7, and mouse TCMK-1 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SMC6 antibody A300-237A-T (lot A300-237A-T-3) used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of Human SMC6 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SMC6 antibody A300-237A-T (lot A300-237A-T-3) used for IP at 20 µl per reaction. For blotting immunoprecipitated SMC6, A300-237A-M was used at 1:1000. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of human SMC6 by immunohistochemistry. Sample: FFPE section of prostate carcinoma. Antibody: Rabbit anti-SMC6 antibody (A300-237A-T-3) used at 1:40. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P).
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