Antibody was affinity purified using an epitope specific to Cul7 immobilized on solid support. The epitope recognized by A300-223A-M maps to a region between residues 1500 and 1550 of human Cullin homolog 7 using the numbering given in SwissProt entry Q14999 (GeneID 9820).
3M1, CUL-7, cullin-7, dJ20C7.5, KIAA0076
between 1500 and 1550
2 - 8°C
Detection of human and mouse Cul7 by western blot. Samples: Whole cell lysate (50 µg) from Hep-G2, HEK293T, HeLa, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Cul7 antibody A300-223A-M (lot A300-223A-M-4) used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.