Antibody was affinity purified using an epitope specific to RFC4 immobilized on solid support. The epitope recognized by A300-144A-T maps to a region between residues 1 and 50 of human Replication Factor C subunit 4 using the numbering given in entry NP_002907.1 (GeneID 5984). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
A1, A1 37 kDa subunit, activator 1 37 kDa subunit, activator 1 subunit 4, replication factor C (activator 1) 4, 37kDa, replication factor C 37 kDa subunit, replication factor C subunit 4, RFC 37 kDa subunit, RF-C 37 kDa subunit, RFC37
between 1 and 50
2 - 8°C
Detection of human RFC4 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified goat anti-RFC4 antibody A300-144A-T (lot A300-144A-T-2) used for WB at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.