Antibody was affinity purified using an epitope specific to RFC2 immobilized on solid support. The epitope recognized by A300-142A-M maps to a region between residues 1 and 50 of human Replication Factor C subunit 2 using the numbering given in entry NP_852136.1 (GeneID 5982).
A1 40 kDa subunit, Activator 1 40 kDa subunit, activator 1 subunit 2, replication factor C (activator 1) 2, 40kDa, Replication factor C 40 kDa subunit, replication factor C subunit 2, RF-C 40 kDa subunit, RFC40
between 1 and 50
2 - 8°C
Detection of human RFC2 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RFC2 antibody A300-142A-M (lot A300-142A-M-1) used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 3 minutes.