Antibody was affinity purified using an epitope specific to ATR immobilized on solid support. The epitope recognized by A300-137A-M maps to a region between residues 400 and 450 of human Ataxia Telangiectasia and Rad3-related using the numbering given in entry NP_001175.1 (GeneID 545).
ataxia telangiectasia and Rad3-related protein, FCTCS, FRAP-related protein 1, FRAP-related protein-1, FRP1, MEC1, MEC1, mitosis entry checkpoint 1, homolog, SCKL, SCKL1, serine/threonine-protein kinase ATR
between 400 and 450
2 - 8°C
Detection of human ATR by western blot. Samples: Whole cell lysate (50, 15 and 5 µg) from HeLa and (50 µg) from HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ATR antibody A300-137A-M (lot A300-137A-M-2) used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.