Antibody was affinity purified using an epitope specific to EPLIN immobilized on solid support. The epitope recognized by A300-103A-T maps to a region between residues 700 and the C-terminus (residue 759) of human epithelial protein lost in neoplasm beta using the numbering given in entry NP_057441.1 (GeneID 51474)
1110021C24Rik, Epithelial protein lost in neoplasm, epithelial protein lost in neoplasm beta, EPLIN, LDLCQ8, LIM domain and actin-binding protein 1, SREBP3, sterol regulatory element binding protein 3
between 700 and C-term
2 - 8°C
Detection of human EPLIN by western blot. Samples: Whole cell lysate from MCF-7 (5, 15 and 50 µg) cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-EPLIN antibody A300-103A-T (lot A300-103A-T-4) used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.