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HCV Protease FRET Substrate

Catalogue number:
ANA22991-50
Supplier:
Size:
50 mg _$$_
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£24910.00 Estimated delivery Thu 4 Apr
Shipping is calculated in checkout
Purity:

Peak Area by HPLC ≥95%

Molecular Weight:

1548.6

Product Description:

This peptide is a HCV protease substrate incorporating an ester bond between residues P1 and P1. Due to ready transesterification of the scissile bond to the acyl-enzyme intermediate, this substrate shows very high kcat/Km values, enabling detection of activity with subnanomolar nonstructural protein 3 (NS3 protease) concentrations. It is widely used for the continuous assay of NS3 protease activity. Substrate cleavage is proportional to the enzyme concentration with a detection limit for NS3 between 1 nM and 250 pM. Upon cleavage of this substrate, fluorescence can be monitored at Abs/Em = 355/500 nm.

Storage Temperature:

-20°C Darkness

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