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Rabbit anti-Vimentin Antibody, Affinity Purified

Catalogue number:
Size:
20 µg _$$_
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Applications:

WB, IP, IHC

Antibody Host:

Rabbit

Species Reactivity:

Human, Mouse

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

Immunogen:

Between 416 and 466

Alternative Names:

epididymis secretory sperm binding protein, vimentin

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Product Description:

Antibody was affinity purified using an epitope specific to Vimentin immobilized on solid support.The epitope recognized by A301-620A maps to a region between residue 416 and 466 of human vimentin using the numbering given in entry NP_003371.2 (GeneID 7431).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Storage Temperature:

2 - 8°C

Detection of human and mouse Vimentin by
Detection of human and mouse Vimentin by western blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg), HEK293T (T; 50 µg), and mouse NIH 3T3 (M; 50 µg) cells. Antibody: Affinity purified rabbit anti-Vimentin antibody A301-620A used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Detection of human Vimentin by immunohis
Detection of human Vimentin by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- Vimentin (Cat. No. A301-620A Lot1) used at a dilution of 1:200 (1µg/ml). Detection: DAB
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