Antibody was affinity purified using an epitope specific to KIF18A immobilized on solid support. The epitope recognized by A301-079A maps to a region between residue 650 and 700 of human kinesin family member 18A using the numbering given in entry NP_112494.2 (GeneID 81930). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
- Applications: IP
kinesin-like protein KIF18A, Marrow stromal KIF18A, MS-KIF18A, PPP1R99, protein phosphatase 1, regulatory subunit 99
between 650 and 700
2 - 8°C
Detection of human KIF18A by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-KIF18A antibody A301-079A used for IP at 3 µg/mg lysate. KIF18A was also immunoprecipitated by rabbit anti-KIF18A antibody A301-080A, which recognizes a downstream epitope. For blotting immunoprecipitated KIF18A, A301-080A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.