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Rabbit anti-EG5/KIF11 Antibody, Affinity Purified - A301-075A - 20 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-EG5/KIF11 Antibody, Affinity Purified
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Size: 20 µg
£302.00
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Catalogue number:
A301-075A
Name:
Rabbit anti-EG5/KIF11 Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to EG5/KIF11 immobilized on solid support.The epitope recognized by A301-075A maps to a region between residue 925 and 975 of human kinesin-related motor protein Eg5 (kinesin family member 11) using the numbering given in entry NP_004514.2 (GeneID 3832).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP

Product Type:
Antibodies
Alternative Names:
EG5, HKSP, kinesin-like protein 1, kinesin-like protein KIF11, kinesin-like spindle protein HKSP, kinesin-related motor protein Eg5, KNSL1, MCLMR, thyroid receptor-interacting protein 5, TR-interacting protein 5, TRIP5, TRIP-5
Species Reactivity:
Human
Immunogen:
Between 925 and 975
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human EG5/KIF11 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-EG5/KIF11 antibody A301-075A used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. EG5/KIF11 was also immunoprecipitated by rabbit anti-EG5/KIF11 antibody A301-076A, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 seconds (A) and 1 second (B).

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