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Rabbit anti-KIF4A Antibody, Affinity Purified - A301-074A - 20 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-KIF4A Antibody, Affinity Purified
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Size: 20 µg
£302.00
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Catalogue number:
A301-074A
Name:
Rabbit anti-KIF4A Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to KIF4A immobilized on solid support. The epitope recognized by A301-074A maps to a region between residue 1182 and the C-terminus (residue 1232) of human kinesin family member 4A using the numbering given in entry NP_036442.3 (GeneID 24137). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP

Product Type:
Antibodies
Alternative Names:
chromokinesin-A, chromosome-associated kinesin KIF4A, KIF4, KIF4G1, MRX100
Species Reactivity:
Human
Immunogen:
between 1182 and C-term
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human KIF4A by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-KIF4A antibody A301-074A used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. KIF4A was also immunoprecipitated by rabbit anti-KIF4A antibody A301-073A, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).

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