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High Quality Recombinant Rabbit Monoclonal Antibodies
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Rabbit anti-BTAF1 Antibody, Affinity Purified - A301-066A - 100 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-BTAF1 Antibody, Affinity Purified
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Size: 100 µg
£302.00
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Catalogue number:
A301-066A
Name:
Rabbit anti-BTAF1 Antibody, Affinity Purified
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Availability:

Antibody was affinity purified using an epitope specific to BTAF1 immobilized on solid support.The epitope recognized by A301-066A maps to a region between residue 1800 and the C-terminus (residue 1849) of human RNA polymerase II, B-TFIID transcription factor-associated, 170kDa using the numbering given in entry NP_003963.1 (GeneID 9044).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP

Product Type:
Antibodies
Alternative Names:
ATP-dependent helicase BTAF1, BTAF1 RNA polymerase II, B-TFIID transcription factor-associated, 170kDa (Mot1 homolog, S. cerevisiae), B-TFIID transcription factor-associated 170 kDa subunit, MOT1, Mot1 homolog, TAF(II)170, TAF172, TAF-172, TAFII170, TATA-
Species Reactivity:
Human
Immunogen:
Between 1800 and C-term
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human BTAF1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-BTAF1 antibody A301-066A used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. BTAF1 was immunoprecipitated more efficiently by rabbit anti-BTAF1 antibodies A301-063A, A301-064A, and A301-065A, which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).

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