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Rabbit anti-BTAF1 Antibody, Affinity Purified

Catalogue number:
A301-066A
Size:
100 µg
Product is available in:
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Antibody was affinity purified using an epitope specific to BTAF1 immobilized on solid support. The epitope recognized by A301-066A maps to a region between residue 1800 and the C-terminus (residue 1849) of human RNA polymerase II, B-TFIID transcription factor-associated, 170kDa using the numbering given in entry NP_003963.1 (GeneID 9044). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP
Detection of human BTAF1 by western blot
Product Type:

Antibodies

Alternative Names:

ATP-dependent helicase BTAF1, BTAF1 RNA polymerase II, B-TFIID transcription factor-associated, 170kDa (Mot1 homolog, S. cerevisiae), B-TFIID transcription factor-associated 170 kDa subunit, MOT1, Mot1 homolog, TAF(II)170, TAF172, TAF-172, TAFII170, TATA-

Species Reactivity:

Human

Immunogen:

between 1800 and C-term

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human BTAF1 by western blot

Detection of human BTAF1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-BTAF1 antibody A301-066A used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. BTAF1 was immunoprecipitated more efficiently by rabbit anti-BTAF1 antibodies A301-063A, A301-064A, and A301-065A, which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).