Antibody was affinity purified using an epitope specific to TRIM33/TIF1gamma immobilized on solid support. The epitope recognized by A301-058A maps to a region between residue 1 to 50 of human tripartite motif-containing 33 (transcriptional intermediary factor 1 gamma) using the numbering given in entry NP_056990.3 (GeneID 51592). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
- Applications: WB, IP
E3 ubiquitin-protein ligase TRIM33, ECTO, ectodermin homolog, protein Rfg7, PTC7, RET-fused gene 7 protein, RFG7, RING-type E3 ubiquitin transferase TRIM33, TF1G, TIF1G, TIF1GAMMA, TIF1-gamma, TIFGAMMA, Transcription intermediary factor 1-gamma, transcrip
between 1 and 50
2 - 8°C
Detection of human TRIM33/TIF1gamma by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-TRIM33/TIF1gamma antibody A301-058A used for WB at 0.04 µg/ml (A) and 0.1 µg/ml (B). TRIM33/TIF1gamma was immunoprecipitated by rabbit anti-TRIM33/TIF1gamma antibodies A301-059A and A301-060A, which recognize downstream epitopes. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).