Antibody was affinity purified using an epitope specific to ATAD5/FRAG1 immobilized on solid support. The epitope recognized by A301-057A maps to a region between residue 775 and 825 of human ATPase family, AAA domain containing 5 using the numbering given in entry NP_079133.3 (GeneID 79915). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
- Applications: WB, IP
ATPase family AAA domain-containing protein 5, C17orf41, chromosome fragility associated gene 1, chromosome fragility-associated gene 1 protein, ELG1, enhanced level of genomic instability 1 homolog, FRAG1
between 775 and 825
2 - 8°C
Detection of human ATAD5/FRAG1 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa and HEK293T (T; 50 ug) cells. Antibody: Affinity purified rabbit anti-ATAD5/FRAG1 antibody A301-057A used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).