Antibody was affinity purified using an epitope specific to KIF1B immobilized on solid support. The epitope recognized by A301-056A maps to a region between residue 1766 and the C-terminus (residue 1816) of human kinesin family member 1B using the numbering given in entry CAC16629.1 (GeneID 23095). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
- Applications: IP
CMT2, CMT2A, CMT2A1, HMSNII, kinesin superfamily protein KIF1B, kinesin-like protein KIF1B, KLP, NBLST1
between 1766 and C-term
2 - 8°C
Detection of human KIF1B by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-KIF1B antibody A301-056A used for IP at 3 µg/mg lysate. KIF1B was also immunoprecipitated by rabbit anti-KIF1B antibody A301-055A, which recognizes an upstream epitope. For blotting immunoprecipitated KIF1B, A301-055A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.