Antibody was affinity purified using an epitope specific to KIF1B immobilized on solid support. The epitope recognized by A301-055A maps to a region between residue 1575 and 1625 of human kinesin family member 1B using the numbering given in entry CAC16629.1 (GeneID 23095). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
- Applications: WB, IP
CMT2, CMT2A, CMT2A1, HMSNII, kinesin superfamily protein KIF1B, kinesin-like protein KIF1B, KLP, NBLST1
between 1575 and 1625
2 - 8°C
Detection of human and mouse KIF1B by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg) and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-KIF1B antibody A301-055A used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. KIF1B was also immunoprecipitated by rabbit anti-KIF1B antibody A301-056A, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).