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Rabbit anti-ATXN10/SCA10 Antibody, Affinity Purified

Catalogue number:
A301-054A
Size:
20 µg
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Antibody was affinity purified using an epitope specific to ATXN10/SCA10 immobilized on solid support. The epitope recognized by A301-054A maps to a region between residue 425 and 475 of human Ataxin 10 (spinocerebellar ataxia 10) using the numbering given in entry NP_037368.1 (GeneID 25814). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP
Detection of human ATXN10/SCA10 by weste
Product Type:

Antibodies

Alternative Names:

ataxin-10, brain protein E46 homolog, E46L, HUMEEP, SCA10, spinocerebellar ataxia type 10 protein

Species Reactivity:

Human

Immunogen:

between 425 and 475

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human ATXN10/SCA10 by weste

Detection of human ATXN10/SCA10 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-ATXN10/SCA10 antibody A301-054A used for WB at 0.04 µg/ml (A) and 0.1 µg/ml (B) and used for IP at 3 µg/mg lysate. ATXN10/SCA10 was also immunoprecipitated by rabbit anti-ATXN10/SCA10 antibody A301-053A, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).