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Rabbit anti-ARID1B Antibody, Affinity Purified - A301-046A - 20 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-ARID1B Antibody, Affinity Purified
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Size: 20 µg
£302.00
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Catalogue number:
A301-046A
Name:
Rabbit anti-ARID1B Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to ARID1B immobilized on solid support.The epitope recognized by A301-046A maps to a region between residue 1300 and 1350 of human AT rich interactive domain 1B using the numbering given in entry NP_059989.2 (GeneID 57492).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP, IHC

Product Type:
Antibodies
Alternative Names:
6A3-5, ARID domain-containing protein 1B, AT rich interactive domain 1B (SWI1-like), AT-rich interactive domain-containing protein 1B, BAF250B, BRG1-associated factor 250b, BRG1-binding protein ELD/OSA1, BRG1-binding protein hELD/OSA1, BRIGHT, CSS1, DAN15
Species Reactivity:
Human
Immunogen:
Between 1300 and 1350
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human ARID1B by western blot. Samples: Whole cell lysate (15 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ARID1B antibody A301-046A (lot A301-046A-3) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

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Detection of human ARID1B by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ARID1B antibody A301-046A (lot A301-046A-3) used for IP at 3 µg per reaction. ARID1B was also immunoprecipitated by a previous lot of this antibody (lot A301-046A-2) and rabbit anti-ARID1B antibody A301-047A. For blotting immunoprecipitated ARID1B, A301-046A was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

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Detection of human ARID1B by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-ARID1B (Cat. No. A301-046A Lot3) used at a dilution of 1:1,000 (0.2µg/ml). Detection: DAB

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