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Rabbit anti-ATRX Antibody, Affinity Purified

Catalogue number:
A301-045A
Size:
100 µg
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Antibody was affinity purified using an epitope specific to ATRX immobilized on solid support. The epitope recognized by A301-045A maps to a region between residue 2442 and the C-terminus (residue 2492) of human alpha thalassemia/mental retardation syndrome X-linked using the numbering given in entry NP_000480.2 (GeneID 546). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP, IHC
Product Type:

Antibodies

Alternative Names:

alpha thalassemia/mental retardation syndrome X-linked (RAD54 homolog, S. cerevisiae), ATP-dependent helicase ATRX, JMS, MRX52, RAD54, RAD54L, transcriptional regulator ATRX, XH2, X-linked helicase II, X-linked nuclear protein, XNP, ZNF-HX

Species Reactivity:

Human

Immunogen:

between 2442 and C-term

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human ATRX by western blot.

Detection of human ATRX by western blot. Samples: Whole cell lysate (5, 15 and 50 µg) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ATRX antibody A301-045A (lot A301-045A-3) used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human ATRX by western blot

Detection of human ATRX by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ATRX antibody A301-045A (lot A301-045A-3) used for IP at 6 µg per reaction. ATRX was also immunoprecipitated by a previous lot of this antibody (lot A301-045A-2) and rabbit anti-ATRX antibody BL4515 For blotting immunoprecipitated ATRX, A301-045A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human ATRX by immunohistoch

Detection of human ATRX by immunohistochemistry. Sample: FFPE section of human lung cancer. Antibody: Affinity purified rabbit anti-ATRX (Cat. No. A301-045A-3) used at a dilution of 1:1,000 ( 1µg/ml). Detection: DAB