Antibody was affinity purified using an epitope specific to ARID1A/BAF250 immobilized on solid support. The epitope recognized by A301-041A maps to a region between residue 750 and 800 of human AT rich interactive domain 1A (BRG1-associated factor 250kD) using the numbering given in entry NP_006006.3 (GeneID 8289). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
ARID domain-containing protein 1A, AT rich interactive domain 1A (SWI-like), AT-rich interactive domain-containing protein 1A, B120, BAF250, BAF250a, BM029, brain protein 120, BRG1-associated factor 250, BRG1-associated factor 250a, C1orf4, chromatin remo
between 750 and 800
2 - 8°C
Detection of human ARID1A by western blot. Samples: Whole cell lysate (5, 15 and 50 µg) from HeLa cells. Antibody: Affinity purified rabbit anti-ARID1A antibody A301-041A used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.