Antibody was affinity purified using an epitope specific to SMARCC2/BAF170 immobilized on solid support. The epitope recognized by A301-039A maps to a region between residue 1164 and the C-terminus (residue 1214) of human SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily c, member 2 (BRG1-associated factor 170) using the numbering given in entry NP_003066.2 (GeneID 6601). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
BAF170, BRG1-associated factor 170, chromatin remodeling complex BAF170 subunit, CRACC2, mammalian chromatin remodeling complex BRG1-associated factor 170, Rsc8, SWI/SNF complex 170 kDa subunit, SWI/SNF complex subunit SMARCC2, SWI/SNF-related matrix-asso
between 1164 and C-term
2 - 8°C
Detection of human and mouse SMARCC2/BAF170 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-SMARCC2/BAF170 antibody A301-039A used for WB at 0.04 µg/ml (A) and 1 µg/ml (B), and used for IP at 3 µg/mg lysate. SMARCC2/BAF170 was also immunoprecipitated by rabbit anti-SMARCC2/BAF170 antibody A301-038A, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 1 second (A and B).
Detection of human SMARCC2/BAF170 by immunohistochemistry. Sample: FFPE section of human skin basal cell carcinoma. Antibody: Affinity purified rabbit anti- SMARCC2/BAF170 (Cat. No. A301-039A Lot1) used at a dilution of 1:200 (1µg/ml). Detection: DAB