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High Quality Recombinant Rabbit Monoclonal Antibodies
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Rabbit anti-JARID1C Antibody, Affinity Purified - A301-034A - 20 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-JARID1C Antibody, Affinity Purified
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Size: 20 µg
£302.00
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Catalogue number:
A301-034A
Name:
Rabbit anti-JARID1C Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to JARID1C immobilized on solid support.The epitope recognized by A301-034A maps to a region between residue 275 and 325 of human Jumonji, AT rich interactive domain 1C using the numbering given in entry NP_004178.2 (GeneID 8242).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP, ChIP-Seq

Product Type:
Antibodies
Alternative Names:
DXS1272E, histone demethylase JARID1C, JARID1C, JmjC domain-containing protein SMCX, Jumonji, AT rich interactive domain 1C (RBP2-like), Jumonji/ARID domain-containing protein 1C, lysine (K)-specific demethylase 5C, lysine-specific demethylase 5C, MRX13,
Species Reactivity:
Human
Immunogen:
Between 275 and 325
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human and mouse Jarid1C by western blot. Samples: Whole cell lysate (50, 15 µg) from HeLa and (50 µg) from HEK293T, Jurkat, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Jarid1C antibody A301-034A (lot A301-034A-2) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

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Detection of human Jarid1C by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Jarid1C antibody A301-034A (lot A301-034A-2) used for IP at 6 µg per reaction. Jarid1C was also immunoprecipitated by rabbit anti-Jarid1C antibody A301-035A. For blotting immunoprecipitated Jarid1C, A301-034A was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

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Localization of Jarid1C Binding Sites by ChIP-sequencing. Chromatin from K562 cells was immunoprecipitated with anti-Jarid1C antibody A301-034A and analyzed by DNA sequencing. The figure illustrates the peak distribution of Jarid1C binding within a 500 Kb region of chromosome 1 as detected using anti-Jarid1C antibody A301-034A. ChIP-seq validation performed by Diogenode, Denville, NJ.

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