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  2. Bethyl Laboratories, Inc.
  3. Rabbit anti-JARID1C Antibody, Affinity Purified
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Rabbit anti-JARID1C Antibody, Affinity Purified

Catalogue number:
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A301-034A
Supplier:
Bethyl Laboratories, Inc.
Size:
100 µl (200 µg/ml) _$$_
Product is available in:
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  • Ireland
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£436.00
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Estimated delivery Fri 26 Apr
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Applications:

WB, IP, ChIP-Seq

Antibody Host:

Rabbit

Target:

JARID1C

Species Reactivity:

Human

Antibody Type:

Polyclonal

Immunogen:

between 275 and 325

Alternative Names:

lysine-specific demethylase 5C; Smcy homolog, X-linked; Smcx homolog, X chromosome; SMCX; Protein Xe169; Protein SmcX; MRXSJ; MRXSCJ; XE169; DXS1272E; MRXJ; histone demethylase JARID1C; JARID1C; JmjC domain-containing protein SMCX; Jumonji, AT rich intera

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Product Description:

Rabbit anti-JARID1C Antibody, Affinity Purified - 100 µl (200 µg/ml)

Storage Temperature:

2 - 8°C

Detection of human and mouse Jarid1C by
Detection of human and mouse Jarid1C by western blot. Samples: Whole cell lysate (50, 15 µg) from HeLa and (50 µg) from HEK293T, Jurkat, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Jarid1C antibody A301-034A (lot A301-034A-2) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of human Jarid1C by western bl
Detection of human Jarid1C by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Jarid1C antibody A301-034A (lot A301-034A-2) used for IP at 6 µg per reaction. Jarid1C was also immunoprecipitated by rabbit anti-Jarid1C antibody A301-035A. For blotting immunoprecipitated Jarid1C, A301-034A was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Localization of Jarid1C Binding Sites by
Localization of Jarid1C Binding Sites by ChIP-sequencing. Chromatin from K562 cells was immunoprecipitated with anti-Jarid1C antibody A301-034A and analyzed by DNA sequencing. The figure illustrates the peak distribution of Jarid1C binding within a 500 Kb region of chromosome 1 as detected using anti-Jarid1C antibody A301-034A. ChIP-seq validation performed by Diogenode, Denville, NJ.
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