Antibody was affinity purified using an epitope specific to AATF/Che-1 immobilized on solid support. The epitope recognized by A301-032A maps to a region between residue 510 and the C-terminus (residue 560) of human apoptosis antagonizing transcription factor (Che-1) using the numbering given in entry NP_036270.1 (GeneID 26574). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
- Applications: IP
Apoptosis-antagonizing transcription factor, BFR2, CHE1, CHE-1, DED, protein AATF, rb-binding protein Che-1
between 510 and C-term
2 - 8°C
Detection of human AATF/Che-1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-AATF/Che-1 antibody A301-032A used for IP at 3 µg/mg lysate. AATF/Che-1 was also immunoprecipitated by rabbit anti-AATF/Che-1 antibody A301-031A, which recognizes an upstream epitopes For blotting immunoprecipitated AATF/Che-1, A301-031A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.