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Rabbit anti-AATF/Che-1 Antibody, Affinity Purified

Catalogue number:
A301-032A
Size:
100 µg
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Antibody was affinity purified using an epitope specific to AATF/Che-1 immobilized on solid support. The epitope recognized by A301-032A maps to a region between residue 510 and the C-terminus (residue 560) of human apoptosis antagonizing transcription factor (Che-1) using the numbering given in entry NP_036270.1 (GeneID 26574). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: IP
Detection of human AATF/Che-1 by western
Product Type:

Antibodies

Alternative Names:

Apoptosis-antagonizing transcription factor, BFR2, CHE1, CHE-1, DED, protein AATF, rb-binding protein Che-1

Species Reactivity:

Human

Immunogen:

between 510 and C-term

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human AATF/Che-1 by western

Detection of human AATF/Che-1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-AATF/Che-1 antibody A301-032A used for IP at 3 µg/mg lysate. AATF/Che-1 was also immunoprecipitated by rabbit anti-AATF/Che-1 antibody A301-031A, which recognizes an upstream epitopes For blotting immunoprecipitated AATF/Che-1, A301-031A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.