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Rabbit anti-APC5 Antibody, Affinity Purified - A301-026A - 100 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-APC5 Antibody, Affinity Purified
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Size: 100 µg
£302.00
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Catalogue number:
A301-026A
Name:
Rabbit anti-APC5 Antibody, Affinity Purified
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Availability:

Antibody was affinity purified using an epitope specific to APC5 immobilized on solid support.The epitope recognized by A301-026A maps to a region between residue 705 and the C-terminus (residue 755) of human anaphase promoting complex subunit 5 using the numbering given in entry NP_057321.2 (GeneID 51433).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP

Product Type:
Antibodies
Alternative Names:
anaphase-promoting complex subunit 5, APC5, cyclosome subunit 5
Species Reactivity:
Human, Mouse
Immunogen:
Between 705 and C-term
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human APC5 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-APC5 antibody A301-026A (lot A301-026A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

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Detection of mouse APC5 by western blot. Samples: Whole cell lysate (50 µg) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-APC5 antibody A301-026A (lot A301-026A-2) used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

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Detection of human APC5 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-APC5 antibody A301-026A (lot A301-026A-2) used for IP at 3 µg per reaction. APC5 was also immunoprecipitated by a previous lot of this antibody (lot A301-026A-1) and rabbit anti-APC5 antibody BL4442. For blotting immunoprecipitated APC5, A301-026A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

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