Antibody was affinity purified using an epitope specific to PNN/Pinin immobilized on solid support. The epitope recognized by A301-022A maps to a region between residue 667 and the C-terminus (residue 717) of human Pinin, desmosome associated protein using the numbering given in entry NP_002678.2 (GeneID 5411). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
140 kDa nuclear and cell adhesion-related phosphoprotein, desmosome-associated protein, domain-rich serine protein, DRS, DRS protein, DRSP, melanoma metastasis clone A protein, memA, neutrophil protein, nuclear protein SDK3, pinin, SDK3, SR-like protein
between 667 and C-term
2 - 8°C
Detection of human and mouse PNN/Pinin by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg) and mouse NIH 3T3 (M; 50 µg) cells. Antibody: Affinity purified rabbit anti-PNN/Pinin antibody A301-022A used for WB at 0.1 µg/ml (A) and 1 µg/ml (B), and used for IP at 3 µg/mg lysate. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).