Antibody was affinity purified using an epitope specific to SMARCC1/BAF155 immobilized on solid support. The epitope recognized by A301-020A maps to a region between residue 750 and 800 of human SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily c, member 1/BRG1-associated factor 155 using the numbering given in entry NP_003065.2 (GeneID 6599). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
BAF155, BRG1-associated factor 155, chromatin remodeling complex BAF155 subunit, CRACC1, mammalian chromatin remodeling complex BRG1-associated factor 155, Rsc8, SRG3, SWI/SNF complex 155 kDa subunit, SWI/SNF complex subunit SMARCC1, SWI/SNF-related matri
between 750 and 800
2 - 8°C
Detection of human SMARCC1/BAF155 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-SMARCC1/BAF155 antibody A301-020A used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. SMARCC1/BAF155 was also immunoprecipitated by rabbit anti-SMARCC1/BAF155 antibodies A301-019A and A301-021A, which recognize other epitopes. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).