Antibody was affinity purified using an epitope specific to SMARCA2/BRM immobilized on solid support. The epitope recognized by A301-015A maps to a region between residue 100 and 150 of human SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2 using the numbering given in entry CAA51407.1 (GeneID 6595). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
ATP-dependent helicase SMARCA2, BAF190, BAF190B, brahma homolog, BRG1-associated factor 190B, BRM, global transcription activator homologous sequence, hBRM, hSNF2a, NCBRS, probable global transcription activator SNF2L2, protein brahma homolog, SNF2, SNF2/
between 100 and 150
2 - 8°C
Detection of human SMARCA2/BRM by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-SMARCA2/BRM antibody A301-015A used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. SMARCA2/BRM was also immunoprecipitated by rabbit anti-SMARCA2/BRM antibodies A301-014A and A301-016A, which recognize other epitopes. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 3 seconds (B).