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Rabbit anti-PAPOLA Antibody, Affinity Purified

Catalogue number:
A301-010A
Size:
20 µg
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Antibody was affinity purified using an epitope specific to PAPOLA immobilized on solid support. The epitope recognized by A301-010A maps to a region between residue 694 and the C-terminus (residue 744) of human poly(A) polymerase alpha using the numbering given in entry NP_116021.2 (GeneID 10914). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP
Product Type:

Antibodies

Alternative Names:

PAP, PAP-alpha, poly(A) polymerase alpha, polynucleotide adenylyltransferase alpha

Species Reactivity:

Human

Immunogen:

between 694 and C-term

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human PAPOLA by western blo

Detection of human PAPOLA by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibody: Affinity purified rabbit anti-PAPOLA antibody A301-010A used for WB at 0.04 µg/ml (A) and 1 µg/ml (B). PAPOLA was immunoprecipitated by rabbit anti-PAPOLA antibodies A301-008A and A301-009A, which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).