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Rabbit anti-PAPOLA Antibody, Affinity Purified

Catalogue number:
A301-009A
Size:
100 µg
Product is available in:
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Antibody was affinity purified using an epitope specific to PAPOLA immobilized on solid support. The epitope recognized by A301-009A maps to a region between residue 600 and 650 of human poly(A) polymerase alpha using the numbering given in entry NP_116021.2 (GeneID 10914). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: IP
Product Type:

Antibodies

Alternative Names:

PAP, PAP-alpha, poly(A) polymerase alpha, polynucleotide adenylyltransferase alpha

Species Reactivity:

Human

Immunogen:

between 600 and 650

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human PAPOLA by western blo

Detection of human PAPOLA by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibody: Affinity purified rabbit anti-PAPOLA antibody A301-009A used for IP at 3 µg/mg lysate. PAPOLA was also immunoprecipitated by rabbit anti-PAPOLA antibody A301-008A, which recognizes an upstream epitope. For blotting immunoprecipitated PAPOLA, rabbit anti-PAPOLA antibody A301-010A was used at 1 µg/ml,. Detection: Chemiluminescence with an exposure time of 10 seconds.