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Rabbit anti-Periplakin/PPL Antibody, Affinity Purified

Catalogue number:
A301-005A
Size:
20 µg
Product is available in:
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Antibody was affinity purified using an epitope specific to Periplakin/PPL immobilized on solid support. The epitope recognized by A301-005A maps to a region between residue 1150 and 1200 of human periplakin using the numbering given in entry NP_002696.3 (GeneID 5493). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP
Product Type:

Antibodies

Alternative Names:

190 kDa paraneoplastic pemphigus antigen, 195 kDa cornified envelope precursor protein, periplakin

Species Reactivity:

Human, Mouse, Rat

Immunogen:

between 1150 and 1200

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human Periplakin/PPL by wes

Detection of human Periplakin/PPL by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibody: Affinity purified rabbit anti-Periplakin/PPL antibody A301-005A used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). Periplakin/PPL was also immunoprecipitated by rabbit anti-Periplakin/PPL antibody BL4370, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A and B).

Detection of mouse and rat Periplakin/PP

Detection of mouse and rat Periplakin/PPL by western blot. Samples: Whole cell lysate (50 µg) from NIH 3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells. Antibodies: Affinity purified rabbit anti-Periplakin/PPL antibody A301-005A (lot A301-005A-1) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.