Antibody was affinity purified using an epitope specific to Periplakin/PPL immobilized on solid support. The epitope recognized by A301-005A maps to a region between residue 1150 and 1200 of human periplakin using the numbering given in entry NP_002696.3 (GeneID 5493). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
190 kDa paraneoplastic pemphigus antigen, 195 kDa cornified envelope precursor protein, periplakin
Human, Mouse, Rat
between 1150 and 1200
2 - 8°C
Detection of human Periplakin/PPL by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibody: Affinity purified rabbit anti-Periplakin/PPL antibody A301-005A used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). Periplakin/PPL was also immunoprecipitated by rabbit anti-Periplakin/PPL antibody BL4370, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A and B).
Detection of mouse and rat Periplakin/PPL by western blot. Samples: Whole cell lysate (50 µg) from NIH 3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells. Antibodies: Affinity purified rabbit anti-Periplakin/PPL antibody A301-005A (lot A301-005A-1) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.