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Rabbit anti-Periplakin/PPL Antibody, Affinity Purified - A301-005A - 20 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-Periplakin/PPL Antibody, Affinity Purified
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Size: 20 µg
£302.00
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Catalogue number:
A301-005A
Name:
Rabbit anti-Periplakin/PPL Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to Periplakin/PPL immobilized on solid support.The epitope recognized by A301-005A maps to a region between residue 1150 and 1200 of human periplakin using the numbering given in entry NP_002696.3 (GeneID 5493).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP

Product Type:
Antibodies
Alternative Names:
190 kDa paraneoplastic pemphigus antigen, 195 kDa cornified envelope precursor protein, periplakin
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Between 1150 and 1200
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human Periplakin/PPL by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibody: Affinity purified rabbit anti-Periplakin/PPL antibody A301-005A used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). Periplakin/PPL was also immunoprecipitated by rabbit anti-Periplakin/PPL antibody BL4370, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A and B).

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Detection of mouse and rat Periplakin/PPL by western blot. Samples: Whole cell lysate (50 µg) from NIH 3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells. Antibodies: Affinity purified rabbit anti-Periplakin/PPL antibody A301-005A (lot A301-005A-1) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

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