Antibody was affinity purified using an epitope specific to BIG2/ARFGEF2 immobilized on solid support. The epitope recognized by A301-004A maps to a region between residue 300 and 350 of human brefeldin A-inhibited 2 (ADP-ribosylation factor guanine nucleotide-exchange factor 2) using the numbering given in entry NP_006411.1 (GeneID 10564). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
ADP-ribosylation factor guanine nucleotide-exchange factor 2, ADP-ribosylation factor guanine nucleotide-exchange factor 2 (brefeldin A-inhibited), BIG2, brefeldin A-inhibited GEP 2, brefeldin A-inhibited guanine nucleotide-exchange protein 2, dJ1164I10.1
between 300 and 350
2 - 8°C
Detection of human BIG2/ARFGEF2 by western blot and immunoprecipitation. Samples: Whole cell lysate (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibody: Affinity purified rabbit anti-BIG2/ARFGEF2 antibody A301-004A used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). BIG2/AFGEF2 was also immunoprecipitated by rabbit anti-BIG2/ARFGEF2 antibody BL4399, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).