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Rabbit anti-BIG2/ARFGEF2 Antibody, Affinity Purified - A301-004A - 20 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-BIG2/ARFGEF2 Antibody, Affinity Purified
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Size: 20 µg
£302.00
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Catalogue number:
A301-004A
Name:
Rabbit anti-BIG2/ARFGEF2 Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to BIG2/ARFGEF2 immobilized on solid support. The epitope recognized by A301-004A maps to a region between residue 300 and 350 of human brefeldin A-inhibited 2 (ADP-ribosylation factor guanine nucleotide-exchange factor 2) using the numbering given in entry NP_006411.1 (GeneID 10564). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP, IHC

Product Type:
Antibodies
Alternative Names:
ADP-ribosylation factor guanine nucleotide-exchange factor 2, ADP-ribosylation factor guanine nucleotide-exchange factor 2 (brefeldin A-inhibited), BIG2, brefeldin A-inhibited GEP 2, brefeldin A-inhibited guanine nucleotide-exchange protein 2, dJ1164I10.1
Species Reactivity:
Human
Immunogen:
between 300 and 350
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human BIG2/ARFGEF2 by western blot and immunoprecipitation. Samples: Whole cell lysate (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibody: Affinity purified rabbit anti-BIG2/ARFGEF2 antibody A301-004A used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). BIG2/AFGEF2 was also immunoprecipitated by rabbit anti-BIG2/ARFGEF2 antibody BL4399, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).

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Detection of human BIG2/ARFGEF2 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-BIG2/ARFGEF2 (Cat. No. A301-004A) used at a dilution of 1:200 (1µg/ml). Detection: DAB

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