Antibody was affinity purified using an epitope specific to ACINUS/ACN1 immobilized on solid support. The epitope recognized by A300-999A maps to a region between residue 1291 and the C-terminus (residue 1341) of human apoptotic chromatin condensation inducer 1 using the numbering given in entry NP_055792.1 (GeneID 22985). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
ACINUS, ACN, apoptotic chromatin condensation inducer in the nucleus, fSAP152, functional spliceosome-associated protein 152
between 1291 and C-term
2 - 8°C
Detection of human ACINUS/ACIN1 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibody: Affinity purified rabbit anti-ACINUS/ACIN1 antibody A300-999A used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).