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Rabbit anti-BIG1/ARFGEF1 Antibody, Affinity Purified - A300-998A - 100 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-BIG1/ARFGEF1 Antibody, Affinity Purified
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Size: 100 µg
£302.00
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Catalogue number:
A300-998A
Name:
Rabbit anti-BIG1/ARFGEF1 Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to BIG1/ARFGEF1 immobilized on solid support.The epitope recognized by A300-998A maps to a region between residue 1800 and the C-terminus (residue 1849) of human brefeldin A-inhibited guanine nucleotide-exchange protein 1 (ADP-ribosylation factor guanine nucleotide-exchange factor 1) using the numbering given in entry NP_006412.2 (GeneID 10565).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP, IHC

Product Type:
Antibodies
Alternative Names:
ADP-ribosylation factor guanine nucleotide-exchange factor 1, ADP-ribosylation factor guanine nucleotide-exchange factor 1 (brefeldin A-inhibited), ARFGEP1, BIG1, Brefeldin A-inhibited GEP 1, brefeldin A-inhibited guanine nucleotide-exchange protein 1, P2
Species Reactivity:
Human, Mouse
Immunogen:
Between 1800 and C-term
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human and mouse BIG1/ARFGEF1 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg) and mouse NIH 3T3 (M; 50 µg) cells. Antibody: Affinity purified rabbit anti-BIG1/ARFGEF1 antibody A300-998A used for WB at 0.1 µg/ml (A) and 1 µg/ml (B), and used for IP at 3 µg/mg lysate. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).

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Detection of human BIG1/ARFGEF1 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-BIG1/ARFGEF1 (Cat. No. A300-998A Lot1) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB

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