Antibody was affinity purified using an epitope specific to SF3b155/SAP155 immobilized on solid support. The epitope recognized by A300-997A maps to a region between residue 200 and 250 of human splicing factor 3b, subunit 1, 155kDa using the numbering given in entry NP_036565.2 (GeneID 23451). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Hsh155, MDS, pre-mRNA processing 10, pre-mRNA splicing factor SF3b, 155 kDa subunit, Pre-mRNA-splicing factor SF3b 155 kDa subunit, PRP10, PRPF10, SAP 155, SAP155, SF3b155, spliceosome-associated protein 155, splicing factor 3B subunit 1, splicing factor
between 200 and 250
2 - 8°C
Detection of human SF3b155/SAP155 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-SF3b155/SAP155 antibody A300-997A used for WB at 0.04 µg/ml (A) and 0.1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). SF3b155/SAP155 was also immunoprecipitated by rabbit anti-SF3b155/SAP155 antibody A300-996A, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure time of 3 seconds (A and B).
Detection of human SF3b155/SAP155 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- SF3b155/SAP155 (Cat. No. A300-997A Lot1) used at a dilution of 1:200 (1µg/ml). Detection: DAB